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首页> 外文OA文献 >Flow cytometric detection of gamma interferon can effectively discriminate Mycobacterium bovis BCG-vaccinated cattle from M. bovis-infected cattle
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Flow cytometric detection of gamma interferon can effectively discriminate Mycobacterium bovis BCG-vaccinated cattle from M. bovis-infected cattle

机译：γ干扰素的流式细胞术检测可以有效地区分牛分枝杆菌BCG接种的牛和牛分枝杆菌感染的牛

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摘要

Mycobacterium bovis is the causative agent of bovine tuberculosis, a disease that is increasing in incidence in United Kingdom cattle herds. In addition to increasing economic losses, the rise in bovine tuberculosis poses a human health risk. There is an urgent requirement for effective strategies for disease eradication; this will likely involve vaccination in conjunction with current test and slaughter policies. A policy involving vaccination would require an accurate diagnosis of M. bovis-infected animals and the potential to distinguish these animals from vaccinates. Currently used diagnostic tests, the skin test and gamma interferon (IFN-gamma) blood test, have a sensitivity of up to 95%. A further complication is that M. bovis BCG-vaccinated animals are also scored positive by these tests. We tested the hypothesis that the quantification of IFN-gamma-producing lymphocytes by flow cytometric analysis of intracellular IFN-gamma expression would provide a more accurate discrimination of M. bovis-infected animals from BCG vaccinates. Significant numbers of IFN-gamma-expressing CD4+ T cells were detected following culture of heparinized blood from M. bovis-infected animals, but not from BCG vaccinates, with purified protein derived from M. bovis (PPD-B) or live mycobacteria. Only 1 of 17 BCG-vaccinated animals had a significant number of CD4+ T lymphocytes expressing IFN-gamma, compared with 21/22 M. bovis-infected animals. This assay could allow an accurate diagnosis of M. bovis and allow the discrimination of BCG-vaccinated cattle from infected cattle.
机译：牛分枝杆菌是牛结核的病原体，这种病在英国牛群中的发病率正在增加。除增加经济损失外，牛结核病的上升还对人类健康构成威胁。迫切需要有效的疾病根除策略；这可能需要结合当前的测试和屠宰政策进行疫苗接种。一项涉及疫苗接种的政策将需要对牛分枝杆菌感染的动物进行准确诊断，并有可能将这些动物与接种疫苗区分开。当前使用的诊断测试，皮肤测试和伽玛干扰素（IFN-γ）血液测试的灵敏度高达95％。进一步的并发症是，牛分枝杆菌BCG疫苗接种的动物也被这些测试评为阳性。我们测试了以下假设，即通过流式细胞术分析细胞内IFN-γ表达对产生IFN-γ的淋巴细胞进行定量，可以从BCG疫苗中更准确地区分牛分枝杆菌感染的动物。在用牛分枝杆菌感染的动物（PPD-B）或活的分枝杆菌培养的牛分枝杆菌感染动物的肝素化血培养后，检测到大量表达IFN-γ的CD4 + T细胞，而不是从卡介苗中接种。与21/22牛牛莫拉氏菌感染的动物相比，接种BCG的17只动物中只有1只具有大量表达IFN-γ的CD4 + T淋巴细胞。该测定法可以准确诊断牛分枝杆菌，并可以将接种卡介苗的牛与感染牛区分开。

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    Sopp, P; Howard, C J; Hope, J C;

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  • 年度 2006
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  • 原文格式 PDF
  • 正文语种 {"code":"en","name":"English","id":9}
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